Abstract: The brain is an incredibly complex organ, with more than 86 billion neurons. Optogenetics has opened a window into the brain and provided an incredible tool for optical studies. However, many structures cannot be seen with diffraction limited imaging. For example, morphological changes in dendritic spines have been linked to memory formation, learning and Alzheimers. In this talk, I will discuss two super-resolution techniques: stimulated emission depletion microscopy (STED) and structured illumination microscopy (SIM). STED requires fiber-coupled excitation and depletion beams to enable studies of freely behaving animals. Adaptive optical elements combined with structured illumination microscopy enable three-dimensional imaging at depth.
Bio: Juliet Gopinath is the Alfred T. and Betty E. Look Professor of Electrical, Computer and Energy Engineering and Physics at the University of Colorado Boulder. She received her B.S. degree in Electrical Engineering from the University of Minnesota and her M.S. and Ph.D. degrees at MIT. She was a member of technical staff at MIT Lincoln Laboratory from 2005 to 2009. Since then, for the past twelve years, she has led a research group at the University of Colorado Boulder. Her current research interests include ultrafast lasers, nonlinear optics, mid-infrared materials, spectroscopy, orbital angular momentum and adaptive optical devices. . She has published 70 peer-reviewed journal articles and over 86 conference presentations. She is the recipient of an R&D 100 Award (2012) and is an OSA Fellow. She served as an Associate Editor for the IEEE Photonics Society Journal (2011-2017), the Associate Director for Cubit (2019), and current is an Associate Editor for Optica.