New Approaches for Visualizing Single-Molecules In Action
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Date: 10-18-2006
Start Time:
4:00pm
End Time: 5:00pm
Speaker: Eric C. Greene
From:
Columbia University
Location: Interschool Lab, 7th floor, Schapiro/CEPSR
Hosted by:
James Yardley - CISE
Abstract:
We use total internal reflection fluorescence microscopy (TIRFM) to directly visualize single proteins as they interact with individual molecule of DNA, and we are specifically interested in proteins that are involved in the repair of damaged DNA. For these studies we have developed new technologies that rely upon fluid lipid bilayers and microscale diffusion barriers to organize DNA molecules into defined "curtains" on the surface of a flow chamber. This "highthroughput" single-molecule approach allows us to visualize hundreds of individual DNA molecules in a single experiment and track the behavior of potentially thousands of individual proteins.