February 16, 2012
Hosted by: Profs. A. Califano and K. Shepard
Speaker: Harris Wang , Ph.D. (Harvard University)
High-precision genome engineering in living cells enables the dissection of complex genetic interactions and the construction of synthetic circuits with increasing sophistication. Multiplex Automated Genome Engineering (MAGE) is a new framework for manipulating chromosomes in living cells at a scale and throughput that far exceeds those of standard recombinant techniques. Here, I will describe the development and application of MAGE to direct genome evolution, uncover epistasis in metabolic networks, and rewire the genetic code. These advances are extended to program communities of cells toward engineering synthetic ecosystems. Efforts to build circuits, cells and communities by multiplexed engineering will shed new light on the governing principles of natural biology and provide design principles for engineered biology.
Harris Wang, PhD is currently at the Wyss Institute for Biologically Inspired Engineering, Harvard University and the Department of Systems Biology, Harvard Medical School.